Reduction of Soybean Meal Non-Starch Polysaccharides and [alpha]-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments

Soybean meal (SBM) is an important protein source in animal feed. However, the levels of SBM inclusion are restricted in some animal species by the presence of antinutritional factors (ANFs), including non-starch polysaccharides (NSPs) and [alpha]-galactosides (GOSs). The aim of this study was to re...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2012-09, Vol.7 (9), p.e44783
Main Authors: Opazo, Rafael, Ortúzar, Felipe, Navarrete, Paola, Espejo, Romilio, Romero, Jaime
Format: Article
Language:English
Subjects:
Bacteria
Feed industry
Fermentation
Polysaccharides
Silage
Soybeans
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page
container_issue 9
container_start_page e44783
container_title PloS one
container_volume 7
creator Opazo, Rafael
Ortúzar, Felipe
Navarrete, Paola
Espejo, Romilio
Romero, Jaime
description Soybean meal (SBM) is an important protein source in animal feed. However, the levels of SBM inclusion are restricted in some animal species by the presence of antinutritional factors (ANFs), including non-starch polysaccharides (NSPs) and [alpha]-galactosides (GOSs). The aim of this study was to reduce the soybean meal NSPs and GOSs by solid-state fermentation (SSF) using a combination of cellulolytic bacteria isolated from different environments (termites, earthworms, corn silage and bovine ruminal content). To analyse the key enzymatic activities, the isolates were grown in minimal media containing NSPs extracted from SBM. The selected bacterial strains belonged to the genera Streptomyces, Cohnella and Cellulosimicrobium. SSF resulted in a reduction of nearly 24% in the total NSPs, 83% of stachyose and 69% of raffinose and an increase in the protein content. These results suggest that cellulolytic bacteria-based SSF processing facilitates SBM nutritional improvement. In addition, the use of fermented SBM in animal diets can be recommended.
doi_str_mv 10.1371/journal.pone.0044783
format article
fullrecord <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A543313566</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A543313566</galeid><sourcerecordid>A543313566</sourcerecordid><originalsourceid>FETCH-LOGICAL-g1666-a16f5eb3fac9546cbb9398c608f306498e198b486a363a847dc9977b4cbf2a313</originalsourceid><addsrcrecordid>eNqNkU1P4zAQhiMEEl_7D_ZgaaWV9pCS1ImTHNnyKQFdUeCCUDV2xo2Ra1e2g-hf4lfishxaiQOaw4zs53kPM0nyM88GOa3yo2fbOwN6sLAGB1lWFFVNt5K9vKHDlA0zur027yb73j9nWUlrxvaSt1tsexGUNcRKMrFLjmDINYImN9akkwBOdOSf1UsPQnTgVIuegGnJI-hFB0_pOWgQwfqPD76MGVq1KzEgOUM3RxPHVf69V2ZGRqh1r2NeUIL8jSY6BWTMAyiDLZHOzsmJkhJdFMmpeVHOmlWIP0x2JGiPPz77QXJ_dno3ukivxueXo-OrdJYzxlLImSyRUwmiKQsmOG9oUwuW1ZJmrGhqzJuaFzUDyijURdWKpqkqXgguh0BzepD8-p87A41TZaQNDsRceTE9LgsaiZKxSA2-oGK1OFciHkKq-L4h_NkQIhPwNcyg9356Obn9Pjt-2GR_r7FdvFzovNX9aud-HXwHnO-tUA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Reduction of Soybean Meal Non-Starch Polysaccharides and [alpha]-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments</title><source>Access via ProQuest (Open Access)</source><source>PubMed Central</source><creator>Opazo, Rafael ; Ortúzar, Felipe ; Navarrete, Paola ; Espejo, Romilio ; Romero, Jaime</creator><creatorcontrib>Opazo, Rafael ; Ortúzar, Felipe ; Navarrete, Paola ; Espejo, Romilio ; Romero, Jaime</creatorcontrib><description>Soybean meal (SBM) is an important protein source in animal feed. However, the levels of SBM inclusion are restricted in some animal species by the presence of antinutritional factors (ANFs), including non-starch polysaccharides (NSPs) and [alpha]-galactosides (GOSs). The aim of this study was to reduce the soybean meal NSPs and GOSs by solid-state fermentation (SSF) using a combination of cellulolytic bacteria isolated from different environments (termites, earthworms, corn silage and bovine ruminal content). To analyse the key enzymatic activities, the isolates were grown in minimal media containing NSPs extracted from SBM. The selected bacterial strains belonged to the genera Streptomyces, Cohnella and Cellulosimicrobium. SSF resulted in a reduction of nearly 24% in the total NSPs, 83% of stachyose and 69% of raffinose and an increase in the protein content. These results suggest that cellulolytic bacteria-based SSF processing facilitates SBM nutritional improvement. In addition, the use of fermented SBM in animal diets can be recommended.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0044783</identifier><language>eng</language><publisher>Public Library of Science</publisher><subject>Bacteria ; Feed industry ; Fermentation ; Polysaccharides ; Silage ; Soybeans</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e44783</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids></links><search><creatorcontrib>Opazo, Rafael</creatorcontrib><creatorcontrib>Ortúzar, Felipe</creatorcontrib><creatorcontrib>Navarrete, Paola</creatorcontrib><creatorcontrib>Espejo, Romilio</creatorcontrib><creatorcontrib>Romero, Jaime</creatorcontrib><title>Reduction of Soybean Meal Non-Starch Polysaccharides and [alpha]-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments</title><title>PloS one</title><description>Soybean meal (SBM) is an important protein source in animal feed. However, the levels of SBM inclusion are restricted in some animal species by the presence of antinutritional factors (ANFs), including non-starch polysaccharides (NSPs) and [alpha]-galactosides (GOSs). The aim of this study was to reduce the soybean meal NSPs and GOSs by solid-state fermentation (SSF) using a combination of cellulolytic bacteria isolated from different environments (termites, earthworms, corn silage and bovine ruminal content). To analyse the key enzymatic activities, the isolates were grown in minimal media containing NSPs extracted from SBM. The selected bacterial strains belonged to the genera Streptomyces, Cohnella and Cellulosimicrobium. SSF resulted in a reduction of nearly 24% in the total NSPs, 83% of stachyose and 69% of raffinose and an increase in the protein content. These results suggest that cellulolytic bacteria-based SSF processing facilitates SBM nutritional improvement. In addition, the use of fermented SBM in animal diets can be recommended.</description><subject>Bacteria</subject><subject>Feed industry</subject><subject>Fermentation</subject><subject>Polysaccharides</subject><subject>Silage</subject><subject>Soybeans</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkU1P4zAQhiMEEl_7D_ZgaaWV9pCS1ImTHNnyKQFdUeCCUDV2xo2Ra1e2g-hf4lfishxaiQOaw4zs53kPM0nyM88GOa3yo2fbOwN6sLAGB1lWFFVNt5K9vKHDlA0zur027yb73j9nWUlrxvaSt1tsexGUNcRKMrFLjmDINYImN9akkwBOdOSf1UsPQnTgVIuegGnJI-hFB0_pOWgQwfqPD76MGVq1KzEgOUM3RxPHVf69V2ZGRqh1r2NeUIL8jSY6BWTMAyiDLZHOzsmJkhJdFMmpeVHOmlWIP0x2JGiPPz77QXJ_dno3ukivxueXo-OrdJYzxlLImSyRUwmiKQsmOG9oUwuW1ZJmrGhqzJuaFzUDyijURdWKpqkqXgguh0BzepD8-p87A41TZaQNDsRceTE9LgsaiZKxSA2-oGK1OFciHkKq-L4h_NkQIhPwNcyg9356Obn9Pjt-2GR_r7FdvFzovNX9aud-HXwHnO-tUA</recordid><startdate>20120911</startdate><enddate>20120911</enddate><creator>Opazo, Rafael</creator><creator>Ortúzar, Felipe</creator><creator>Navarrete, Paola</creator><creator>Espejo, Romilio</creator><creator>Romero, Jaime</creator><general>Public Library of Science</general><scope>IOV</scope><scope>ISR</scope></search><sort><creationdate>20120911</creationdate><title>Reduction of Soybean Meal Non-Starch Polysaccharides and [alpha]-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments</title><author>Opazo, Rafael ; Ortúzar, Felipe ; Navarrete, Paola ; Espejo, Romilio ; Romero, Jaime</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g1666-a16f5eb3fac9546cbb9398c608f306498e198b486a363a847dc9977b4cbf2a313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Bacteria</topic><topic>Feed industry</topic><topic>Fermentation</topic><topic>Polysaccharides</topic><topic>Silage</topic><topic>Soybeans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Opazo, Rafael</creatorcontrib><creatorcontrib>Ortúzar, Felipe</creatorcontrib><creatorcontrib>Navarrete, Paola</creatorcontrib><creatorcontrib>Espejo, Romilio</creatorcontrib><creatorcontrib>Romero, Jaime</creatorcontrib><collection>Opposing Viewpoints Resource Center</collection><collection>Gale in Context: Science</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Opazo, Rafael</au><au>Ortúzar, Felipe</au><au>Navarrete, Paola</au><au>Espejo, Romilio</au><au>Romero, Jaime</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reduction of Soybean Meal Non-Starch Polysaccharides and [alpha]-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments</atitle><jtitle>PloS one</jtitle><date>2012-09-11</date><risdate>2012</risdate><volume>7</volume><issue>9</issue><spage>e44783</spage><pages>e44783-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Soybean meal (SBM) is an important protein source in animal feed. However, the levels of SBM inclusion are restricted in some animal species by the presence of antinutritional factors (ANFs), including non-starch polysaccharides (NSPs) and [alpha]-galactosides (GOSs). The aim of this study was to reduce the soybean meal NSPs and GOSs by solid-state fermentation (SSF) using a combination of cellulolytic bacteria isolated from different environments (termites, earthworms, corn silage and bovine ruminal content). To analyse the key enzymatic activities, the isolates were grown in minimal media containing NSPs extracted from SBM. The selected bacterial strains belonged to the genera Streptomyces, Cohnella and Cellulosimicrobium. SSF resulted in a reduction of nearly 24% in the total NSPs, 83% of stachyose and 69% of raffinose and an increase in the protein content. These results suggest that cellulolytic bacteria-based SSF processing facilitates SBM nutritional improvement. In addition, the use of fermented SBM in animal diets can be recommended.</abstract><pub>Public Library of Science</pub><doi>10.1371/journal.pone.0044783</doi><tpages>e44783</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2012-09, Vol.7 (9), p.e44783
issn 1932-6203
1932-6203
language eng
recordid cdi_gale_infotracmisc_A543313566
source Access via ProQuest (Open Access); PubMed Central
subjects Bacteria
Feed industry
Fermentation
Polysaccharides
Silage
Soybeans
title Reduction of Soybean Meal Non-Starch Polysaccharides and [alpha]-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-11-15T16%3A43%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Reduction%20of%20Soybean%20Meal%20Non-Starch%20Polysaccharides%20and%20%5Balpha%5D-Galactosides%20by%20Solid-State%20Fermentation%20Using%20Cellulolytic%20Bacteria%20Obtained%20from%20Different%20Environments&rft.jtitle=PloS%20one&rft.au=Opazo,%20Rafael&rft.date=2012-09-11&rft.volume=7&rft.issue=9&rft.spage=e44783&rft.pages=e44783-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0044783&rft_dat=%3Cgale%3EA543313566%3C/gale%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-g1666-a16f5eb3fac9546cbb9398c608f306498e198b486a363a847dc9977b4cbf2a313%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A543313566&rfr_iscdi=true