Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods/Deteccao de Campylobacter spp. em carcacas de frango de corte resfriadas e congeladas pelos metodos imunoenzimatico, PCR e PCR em tempo real

In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS[R] 30, Polymerase Chain Rea...

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Published in:Ciência rural 2018-02, Vol.48 (2)
Main Authors: Reis, Luciana Pimenta, Menezes, Liliane Denize Miranda, Lima, Graciela Kunrath, Santos, Ethiene Luiza de Souza, Dorneles, Elaine Maria Seles, de Assis, Debora Cristina Sampaio, Lage, Andrey Pereira, Cancado, Silvana de Vasconcelos, de Figueiredo, Tadeu Chaves
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container_title Ciência rural
container_volume 48
creator Reis, Luciana Pimenta
Menezes, Liliane Denize Miranda
Lima, Graciela Kunrath
Santos, Ethiene Luiza de Souza
Dorneles, Elaine Maria Seles
de Assis, Debora Cristina Sampaio
Lage, Andrey Pereira
Cancado, Silvana de Vasconcelos
de Figueiredo, Tadeu Chaves
description In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS[R] 30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS[R]30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of Campylobacter spp. were observed between the chilled and frozen broiler carcasses. In conclusion, real-time PCR was the most sensitive method for the detection of Campylobacter spp. in chilled or frozen broiler carcasses, which were mainly contaminated by C. jejuni. Key words: C. jejuni, C. coli, detection methods, VIDAS[R]30. Com o objetivo de detectar e identificar Campylobacter spp. em carcacas de frango de corte utilizando tres metodologias distintas - ensaio imunoenzimatico VIDAS[R]30, Reacao em Cadeia da Polimerase (PCR) e PCR em tempo real - foram coletadas e analisadas 43 carcacas de frango resfriadas e 43 congeladas. Quatro carcacas refrigeradas (4,6%) foram consideradas positivas para Campylobacter spp. pelo VIDAS[R]30 e nenhuma amostra positiva foi identificada quando utilizada a tecnica de PCR. Porem, ao analisar as carcacas pela metodologia da PCR em tempo real, foi observada uma maior incidencia de Campylobacter spp., com 45 amostras (52,3%) positivas, sendo que Campylobacter jejuni foi a especie mais frequentemente encontrada nas amostras (88,8%). Nao foi observada diferenca na frequencia do micro-organismo entre carcacas de frangos resfriadas e congeladas. Concluiu-se que a tecnica de PCR em tempo real apresentou maior sensibilidade na deteccao de Campylobacter spp. em carcacas de frangos de corte e que foi encontrada elevada presenca de carcacas contaminadas, especialmente por C. jejuni. Palavras-chave: C. jejuni, C. coli, metodos de deteccao, VIDAS[R] 30.
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Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS[R] 30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS[R]30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of Campylobacter spp. were observed between the chilled and frozen broiler carcasses. In conclusion, real-time PCR was the most sensitive method for the detection of Campylobacter spp. in chilled or frozen broiler carcasses, which were mainly contaminated by C. jejuni. Key words: C. jejuni, C. coli, detection methods, VIDAS[R]30. Com o objetivo de detectar e identificar Campylobacter spp. em carcacas de frango de corte utilizando tres metodologias distintas - ensaio imunoenzimatico VIDAS[R]30, Reacao em Cadeia da Polimerase (PCR) e PCR em tempo real - foram coletadas e analisadas 43 carcacas de frango resfriadas e 43 congeladas. Quatro carcacas refrigeradas (4,6%) foram consideradas positivas para Campylobacter spp. pelo VIDAS[R]30 e nenhuma amostra positiva foi identificada quando utilizada a tecnica de PCR. Porem, ao analisar as carcacas pela metodologia da PCR em tempo real, foi observada uma maior incidencia de Campylobacter spp., com 45 amostras (52,3%) positivas, sendo que Campylobacter jejuni foi a especie mais frequentemente encontrada nas amostras (88,8%). Nao foi observada diferenca na frequencia do micro-organismo entre carcacas de frangos resfriadas e congeladas. Concluiu-se que a tecnica de PCR em tempo real apresentou maior sensibilidade na deteccao de Campylobacter spp. em carcacas de frangos de corte e que foi encontrada elevada presenca de carcacas contaminadas, especialmente por C. jejuni. 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Concluiu-se que a tecnica de PCR em tempo real apresentou maior sensibilidade na deteccao de Campylobacter spp. em carcacas de frangos de corte e que foi encontrada elevada presenca de carcacas contaminadas, especialmente por C. jejuni. 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Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS[R] 30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS[R]30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of Campylobacter spp. were observed between the chilled and frozen broiler carcasses. In conclusion, real-time PCR was the most sensitive method for the detection of Campylobacter spp. in chilled or frozen broiler carcasses, which were mainly contaminated by C. jejuni. Key words: C. jejuni, C. coli, detection methods, VIDAS[R]30. Com o objetivo de detectar e identificar Campylobacter spp. em carcacas de frango de corte utilizando tres metodologias distintas - ensaio imunoenzimatico VIDAS[R]30, Reacao em Cadeia da Polimerase (PCR) e PCR em tempo real - foram coletadas e analisadas 43 carcacas de frango resfriadas e 43 congeladas. Quatro carcacas refrigeradas (4,6%) foram consideradas positivas para Campylobacter spp. pelo VIDAS[R]30 e nenhuma amostra positiva foi identificada quando utilizada a tecnica de PCR. Porem, ao analisar as carcacas pela metodologia da PCR em tempo real, foi observada uma maior incidencia de Campylobacter spp., com 45 amostras (52,3%) positivas, sendo que Campylobacter jejuni foi a especie mais frequentemente encontrada nas amostras (88,8%). Nao foi observada diferenca na frequencia do micro-organismo entre carcacas de frangos resfriadas e congeladas. Concluiu-se que a tecnica de PCR em tempo real apresentou maior sensibilidade na deteccao de Campylobacter spp. em carcacas de frangos de corte e que foi encontrada elevada presenca de carcacas contaminadas, especialmente por C. jejuni. Palavras-chave: C. jejuni, C. coli, metodos de deteccao, VIDAS[R] 30.</abstract><pub>Universidade Federal de Santa Maria</pub><doi>10.1590/0103-8478cr20161034</doi></addata></record>
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title Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods/Deteccao de Campylobacter spp. em carcacas de frango de corte resfriadas e congeladas pelos metodos imunoenzimatico, PCR e PCR em tempo real
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