Evaluation, Validation, and Demonstration of a Total Protein Assay for Application to Biotoxin Fate Studies

This report summarizes efforts to evaluate, optimize, and validate a commercially available analytical assay for the quantitative determination of total protein in aqueous sample matrices. The data generated during this study will be used to support on-going investigations into the fate of biotoxins...

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Bibliographic Details
Main Authors: Morrissey, Kevin M, Schenning, Amanda M, Bevilacqua, Vicky L, Rice, Jeffrey S
Format: Report
Language:English
Subjects:
ALBUMINS
AQUEOUS ENVIRONMENTS
ASSAYING
BATTLESPACE ENVIRONMENT SIMULATION
Biochemistry
BIOTOXIN FATE
BIOTOXINS
BSA(BOVINE SERUM ALBUMIN)
DECONTAMINATION
DEMONSTRATIONS
DETECTION
JSAWM(JOINT SERVICES AGENT WATER MONITOR)
LYSOZYME
MICROPLATE READER
OPTIMIZATION
Pharmacology
PROTEINS
QUANTIZATION
RICIN
SIMULATION
TOTAL PROTEIN
TOXINS AND ANTITOXINS
VALIDATION
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Summary:This report summarizes efforts to evaluate, optimize, and validate a commercially available analytical assay for the quantitative determination of total protein in aqueous sample matrices. The data generated during this study will be used to support on-going investigations into the fate of biotoxins in aqueous environments. The optimized assay was found to be repeatable, reproducible, and not subject to interference by several different water matrices. The method limit of detection was determined to be 0.350 mg/L, and the method limit of quantization was determined to be 1.17 mg/L. The linear range was determined to be 1.00 to 25.0 mg/L of total protein.