Application of a constrained mixture design for lipase production by Penicillium roqueforti ATCC 10110 under solid-state fermentation and using agro-industrial wastes as substrate

Solid state fermentation (SSF) simulates the natural conditions fungal growth, where the amount of water in the reaction medium must be restricted, thus limiting the use of liquid substrate. An analytical strategy to deal with this limitation is the design of blending with constraints. Thus, the obj...

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Bibliographic Details
Published in:Preparative biochemistry & biotechnology 2022-09, Vol.52 (8), p.885-893
Main Authors: de Menezes, Luiz Henrique Sales, Ramos, Marla Rosa Marques Ferreira, Araujo, Sabryna Couto, Santo, Eliezer Luz do Espírito, Oliveira, Polyany Cabral, Tavares, Iasnaia Maria de Carvalho, Santos, Pedro Henrique, Franco, Marcelo, de Oliveira, Julieta Rangel
Format: Article
Language:English
Subjects:
Agricultural wastes
Biotechnology
Cocoa
Constraints
Design
enzyme
Fermentation
Fungi
Industrial wastes
Lipase
Liquid wastes
Mixtures
Moisture content
optimization
Palm oil
Penicillium roqueforti
Residues
Solid state
Solid state fermentation
Solid wastes
statistical design
Substrates
Vegetable oils
Water content
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Summary:Solid state fermentation (SSF) simulates the natural conditions fungal growth, where the amount of water in the reaction medium must be restricted, thus limiting the use of liquid substrate. An analytical strategy to deal with this limitation is the design of blending with constraints. Thus, the objective of the work was to optimize two constrained waste mixtures for the production of lipase by Penicillium roqueforti ATCC 10110 under SSF, using different substrates that combine solid and liquid waste. For this, the best fermentation time was determined through a fermentative profile, afterwards a restricted-mix design with lower and upper limits of the components of mixture I (cocoa residue, solid palm oil residue and liquid palm oil residue) and II (cocoa residue, mango residue and palm oil residue liquid palm) was applied. By means of Pareto and contour graphs, the maximum production points of lipase in mixtures I (6.67 ± 0.34 U g −1 ) and II (6.87 ± 0.35 U g −1 ) were obtained. The restricted mixture design proved to be a promising tool in the production of lipase by P. roqueforti ATCC 10110 under SSF since the use of restrictions is useful when intending to combine solid and liquid residues in fermentation processes.
ISSN:1082-6068
1532-2297
DOI:10.1080/10826068.2021.2004547