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reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from 2002 to 2006
A survey of infectious bronchitis virus (IBV) genotypes in poultry flocks in selected countries in Western Europe was carried out between March 2002 and December 2006. Identification of IBV was by reverse transcriptase-polymerase chain reaction of RNA extracted from oropharyngeal swabs taken from po...
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| Published in: | Avian pathology 2008-06, Vol.37 (3), p.247-257 |
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| creator | Worthington, K.J Currie, R.J.W Jones, R.C |
| description | A survey of infectious bronchitis virus (IBV) genotypes in poultry flocks in selected countries in Western Europe was carried out between March 2002 and December 2006. Identification of IBV was by reverse transcriptase-polymerase chain reaction of RNA extracted from oropharyngeal swabs taken from poultry flocks exhibiting signs of clinical disease thought to be attributable to IBV. Part of the hypervariable S1 gene of IBV was sequenced to differentiate between the various genotypes. During the survey, 4103 samples were processed, of which 2419 (59%) were positive for IBV. The predominant IBV genotypes detected were 793B and Massachusetts. The third and fourth most common genotypes were two new economically important field types: Italy02, and a virus similar to genotypes originally detected in China called QX. Analysis of the partial S1 sequences of the genotypes detected suggested that approximately 50% of all 793B, Massachusetts types and D274 IBVs were identical to the homologous commercially available live vaccines. Since 2004 the prevalence of Italy02 (present in all countries from which samples were received) has been declining in all countries except Spain, where it appeared to be the predominant genotype. Since 2004 an IBV genotype has been detected in Holland, Germany, Belgium and France similar to QX and the incidence has increased. QX was not detected in the United Kingdom or Spain. When detections thought to be attributable to vaccines were removed, the dominant genotype in France and Europe overall was 793B; in Germany, Holland and Belgium, it was QX-like IBV; and in the United Kingdom and Spain the dominant genotype was Italy02. The present study is the first to identify the prevalence of both Italy02 and QX field-type variants in poultry flocks in Western Europe. Several novel genotypes have also been detected. |
| doi_str_mv | 10.1080/03079450801986529 |
| format | article |
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Identification of IBV was by reverse transcriptase-polymerase chain reaction of RNA extracted from oropharyngeal swabs taken from poultry flocks exhibiting signs of clinical disease thought to be attributable to IBV. Part of the hypervariable S1 gene of IBV was sequenced to differentiate between the various genotypes. During the survey, 4103 samples were processed, of which 2419 (59%) were positive for IBV. The predominant IBV genotypes detected were 793B and Massachusetts. The third and fourth most common genotypes were two new economically important field types: Italy02, and a virus similar to genotypes originally detected in China called QX. Analysis of the partial S1 sequences of the genotypes detected suggested that approximately 50% of all 793B, Massachusetts types and D274 IBVs were identical to the homologous commercially available live vaccines. Since 2004 the prevalence of Italy02 (present in all countries from which samples were received) has been declining in all countries except Spain, where it appeared to be the predominant genotype. Since 2004 an IBV genotype has been detected in Holland, Germany, Belgium and France similar to QX and the incidence has increased. QX was not detected in the United Kingdom or Spain. When detections thought to be attributable to vaccines were removed, the dominant genotype in France and Europe overall was 793B; in Germany, Holland and Belgium, it was QX-like IBV; and in the United Kingdom and Spain the dominant genotype was Italy02. The present study is the first to identify the prevalence of both Italy02 and QX field-type variants in poultry flocks in Western Europe. Several novel genotypes have also been detected.</description><identifier>ISSN: 0307-9457</identifier><identifier>EISSN: 1465-3338</identifier><identifier>DOI: 10.1080/03079450801986529</identifier><identifier>PMID: 18568650</identifier><language>eng</language><publisher>England: Taylor & Francis Group</publisher><subject>Animals ; Avian flu ; Chickens - virology ; Coronavirus Infections - epidemiology ; Coronavirus Infections - veterinary ; Coronavirus Infections - virology ; disease detection ; disease prevalence ; epidemiological studies ; Europe - epidemiology ; flocks ; genes ; Genotype ; Genotype & phenotype ; infectious bronchitis ; Infectious bronchitis virus ; Infectious bronchitis virus - classification ; Infectious bronchitis virus - genetics ; live vaccines ; microbial genetics ; molecular sequence data ; nucleotide sequences ; Phylogeny ; Poultry ; Poultry Diseases - epidemiology ; Poultry Diseases - virology ; reverse transcriptase polymerase chain reaction ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; Ribonucleic acid ; RNA ; strains ; Studies ; Time Factors ; Vaccines</subject><ispartof>Avian pathology, 2008-06, Vol.37 (3), p.247-257</ispartof><rights>Copyright Houghton Trust Ltd 2008</rights><rights>Copyright Taylor & Francis Ltd. Jun 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-4d5ce808a146a9448618cf750fb5e571ad41a50a31bb80e1a73e5c31f71648be3</citedby><cites>FETCH-LOGICAL-c560t-4d5ce808a146a9448618cf750fb5e571ad41a50a31bb80e1a73e5c31f71648be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18568650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Worthington, K.J</creatorcontrib><creatorcontrib>Currie, R.J.W</creatorcontrib><creatorcontrib>Jones, R.C</creatorcontrib><title>reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from 2002 to 2006</title><title>Avian pathology</title><addtitle>Avian Pathol</addtitle><description>A survey of infectious bronchitis virus (IBV) genotypes in poultry flocks in selected countries in Western Europe was carried out between March 2002 and December 2006. Identification of IBV was by reverse transcriptase-polymerase chain reaction of RNA extracted from oropharyngeal swabs taken from poultry flocks exhibiting signs of clinical disease thought to be attributable to IBV. Part of the hypervariable S1 gene of IBV was sequenced to differentiate between the various genotypes. During the survey, 4103 samples were processed, of which 2419 (59%) were positive for IBV. The predominant IBV genotypes detected were 793B and Massachusetts. The third and fourth most common genotypes were two new economically important field types: Italy02, and a virus similar to genotypes originally detected in China called QX. Analysis of the partial S1 sequences of the genotypes detected suggested that approximately 50% of all 793B, Massachusetts types and D274 IBVs were identical to the homologous commercially available live vaccines. Since 2004 the prevalence of Italy02 (present in all countries from which samples were received) has been declining in all countries except Spain, where it appeared to be the predominant genotype. Since 2004 an IBV genotype has been detected in Holland, Germany, Belgium and France similar to QX and the incidence has increased. QX was not detected in the United Kingdom or Spain. When detections thought to be attributable to vaccines were removed, the dominant genotype in France and Europe overall was 793B; in Germany, Holland and Belgium, it was QX-like IBV; and in the United Kingdom and Spain the dominant genotype was Italy02. The present study is the first to identify the prevalence of both Italy02 and QX field-type variants in poultry flocks in Western Europe. Several novel genotypes have also been detected.</description><subject>Animals</subject><subject>Avian flu</subject><subject>Chickens - virology</subject><subject>Coronavirus Infections - epidemiology</subject><subject>Coronavirus Infections - veterinary</subject><subject>Coronavirus Infections - virology</subject><subject>disease detection</subject><subject>disease prevalence</subject><subject>epidemiological studies</subject><subject>Europe - epidemiology</subject><subject>flocks</subject><subject>genes</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>infectious bronchitis</subject><subject>Infectious bronchitis virus</subject><subject>Infectious bronchitis virus - classification</subject><subject>Infectious bronchitis virus - genetics</subject><subject>live vaccines</subject><subject>microbial genetics</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>Poultry</subject><subject>Poultry Diseases - epidemiology</subject><subject>Poultry Diseases - virology</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>strains</subject><subject>Studies</subject><subject>Time Factors</subject><subject>Vaccines</subject><issn>0307-9457</issn><issn>1465-3338</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1DAQhiMEotvCD-ACFofeAuPPOBKXqiofUiUOUHGMHO-kTZXEYews5Mwfx6tdCYlKcJrR-HlH7_gtihcc3nCw8BYkVLXSueW1NVrUj4oNV0aXUkr7uNjs38sMVCfFaYz3AGC0Fk-LE261yQLYFL8Id0gRWSI3RU_9nFzEcg7DOiLllvk710-M0PnUh4nFhXa4stCxfupwP1siaylM_q5PfWS7nvLgFqeQ1hljptg3jAlpYlcLhRlZR2FkAkCwFPbVPCuedG6I-PxYz4qb91dfLz-W158_fLq8uC69NpBKtdUeLViXT3S1UtZw67tKQ9dq1BV3W8WdBid521pA7iqJ2kveVdwo26I8K84Pe2cK35dsqhn76HEY3IT5isbUQqqaQwZf_wXeh4Wm7K0RoIyQFZgM8QPkKcRI2DUz9aOjteHQ7ONpHsSTNS-Pi5d2xO0fxTGPDFQHIH9uoNH9CDRsm-TWIVCXE_J9fLi2ST9TVr77r1L-y9mrg7xzoXG3lOmbLwK4BKihFkLJ3_g3ubc</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Worthington, K.J</creator><creator>Currie, R.J.W</creator><creator>Jones, R.C</creator><general>Taylor & Francis Group</general><general>Taylor & Francis Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>200806</creationdate><title>reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from 2002 to 2006</title><author>Worthington, K.J ; Currie, R.J.W ; Jones, R.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-4d5ce808a146a9448618cf750fb5e571ad41a50a31bb80e1a73e5c31f71648be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Avian flu</topic><topic>Chickens - virology</topic><topic>Coronavirus Infections - epidemiology</topic><topic>Coronavirus Infections - veterinary</topic><topic>Coronavirus Infections - virology</topic><topic>disease detection</topic><topic>disease prevalence</topic><topic>epidemiological studies</topic><topic>Europe - epidemiology</topic><topic>flocks</topic><topic>genes</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>infectious bronchitis</topic><topic>Infectious bronchitis virus</topic><topic>Infectious bronchitis virus - classification</topic><topic>Infectious bronchitis virus - genetics</topic><topic>live vaccines</topic><topic>microbial genetics</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>Poultry</topic><topic>Poultry Diseases - epidemiology</topic><topic>Poultry Diseases - virology</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>strains</topic><topic>Studies</topic><topic>Time Factors</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Worthington, K.J</creatorcontrib><creatorcontrib>Currie, R.J.W</creatorcontrib><creatorcontrib>Jones, R.C</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Avian pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Worthington, K.J</au><au>Currie, R.J.W</au><au>Jones, R.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from 2002 to 2006</atitle><jtitle>Avian pathology</jtitle><addtitle>Avian Pathol</addtitle><date>2008-06</date><risdate>2008</risdate><volume>37</volume><issue>3</issue><spage>247</spage><epage>257</epage><pages>247-257</pages><issn>0307-9457</issn><eissn>1465-3338</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>A survey of infectious bronchitis virus (IBV) genotypes in poultry flocks in selected countries in Western Europe was carried out between March 2002 and December 2006. Identification of IBV was by reverse transcriptase-polymerase chain reaction of RNA extracted from oropharyngeal swabs taken from poultry flocks exhibiting signs of clinical disease thought to be attributable to IBV. Part of the hypervariable S1 gene of IBV was sequenced to differentiate between the various genotypes. During the survey, 4103 samples were processed, of which 2419 (59%) were positive for IBV. The predominant IBV genotypes detected were 793B and Massachusetts. The third and fourth most common genotypes were two new economically important field types: Italy02, and a virus similar to genotypes originally detected in China called QX. Analysis of the partial S1 sequences of the genotypes detected suggested that approximately 50% of all 793B, Massachusetts types and D274 IBVs were identical to the homologous commercially available live vaccines. Since 2004 the prevalence of Italy02 (present in all countries from which samples were received) has been declining in all countries except Spain, where it appeared to be the predominant genotype. Since 2004 an IBV genotype has been detected in Holland, Germany, Belgium and France similar to QX and the incidence has increased. QX was not detected in the United Kingdom or Spain. When detections thought to be attributable to vaccines were removed, the dominant genotype in France and Europe overall was 793B; in Germany, Holland and Belgium, it was QX-like IBV; and in the United Kingdom and Spain the dominant genotype was Italy02. The present study is the first to identify the prevalence of both Italy02 and QX field-type variants in poultry flocks in Western Europe. Several novel genotypes have also been detected.</abstract><cop>England</cop><pub>Taylor & Francis Group</pub><pmid>18568650</pmid><doi>10.1080/03079450801986529</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Science and Technology Collection (Reading list) |
| subjects | Animals Avian flu Chickens - virology Coronavirus Infections - epidemiology Coronavirus Infections - veterinary Coronavirus Infections - virology disease detection disease prevalence epidemiological studies Europe - epidemiology flocks genes Genotype Genotype & phenotype infectious bronchitis Infectious bronchitis virus Infectious bronchitis virus - classification Infectious bronchitis virus - genetics live vaccines microbial genetics molecular sequence data nucleotide sequences Phylogeny Poultry Poultry Diseases - epidemiology Poultry Diseases - virology reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - veterinary Ribonucleic acid RNA strains Studies Time Factors Vaccines |
| title | reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from 2002 to 2006 |
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