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Discovery and in vitro Biosynthesis of Haloduracin, a Two-Component Lantibiotic
Lantibiotics are ribosomally synthesized peptides that undergo posttranslational modifications to their mature, antimicrobial form. They are characterized by the unique amino acids lanthionine and methyllanthionine, introduced by means of dehydration of Ser/Thr residues followed by reaction of the r...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 2006-11, Vol.103 (46), p.17243-17248 |
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| container_issue | 46 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | McClerren, Amanda L. Cooper, Lisa E. Quan, Chao Thomas, Paul M. Kelleher, Neil L. van der Donk, Wilfred A. |
| description | Lantibiotics are ribosomally synthesized peptides that undergo posttranslational modifications to their mature, antimicrobial form. They are characterized by the unique amino acids lanthionine and methyllanthionine, introduced by means of dehydration of Ser/Thr residues followed by reaction of the resulting dehydro amino acids with cysteines to form thioether linkages. Two-component lantibiotics use two peptides that are each posttranslationally modified to yield two functionally distinct products that act in synergy to provide bactericidal activity. By using genetic data instead of isolation, a two-component lantibiotic, haloduracin, was identified in the genome of the Gram-positive alkaliphilic bacterium Bacillus halodurans C-125. We show that heterologously expressed and purified precursor peptides HalA1 and HalA2 are processed by the purified modification enzymes HalM1 and HalM2 in an in vitro reconstitution of the biosynthesis of a two-component lantibiotic. The activity of each HalM enzyme is substratespecific, and the assay products exhibit antimicrobial activity after removal of their leader sequences at an engineered Factor Xa cleavage site, indicating that correct thioether formation has occurred. Haloduracin's biological activity depends on the presence of both modified peptides. The structures of the two mature haloduracin peptides Halα and Halβ were investigated, indicating that they have similarities as well as some distinct differences compared with other two-component lantibiotics. |
| doi_str_mv | 10.1073/pnas.0606088103 |
| format | article |
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They are characterized by the unique amino acids lanthionine and methyllanthionine, introduced by means of dehydration of Ser/Thr residues followed by reaction of the resulting dehydro amino acids with cysteines to form thioether linkages. Two-component lantibiotics use two peptides that are each posttranslationally modified to yield two functionally distinct products that act in synergy to provide bactericidal activity. By using genetic data instead of isolation, a two-component lantibiotic, haloduracin, was identified in the genome of the Gram-positive alkaliphilic bacterium Bacillus halodurans C-125. We show that heterologously expressed and purified precursor peptides HalA1 and HalA2 are processed by the purified modification enzymes HalM1 and HalM2 in an in vitro reconstitution of the biosynthesis of a two-component lantibiotic. The activity of each HalM enzyme is substratespecific, and the assay products exhibit antimicrobial activity after removal of their leader sequences at an engineered Factor Xa cleavage site, indicating that correct thioether formation has occurred. Haloduracin's biological activity depends on the presence of both modified peptides. The structures of the two mature haloduracin peptides Halα and Halβ were investigated, indicating that they have similarities as well as some distinct differences compared with other two-component lantibiotics.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0606088103</identifier><identifier>PMID: 17085596</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Acetoacetates - metabolism ; Adducts ; Amino Acid Sequence ; Amino acids ; Antibiotics ; Antimicrobials ; Bacillus - chemistry ; Bacillus - genetics ; Bacillus - metabolism ; Bacillus halodurans ; Bacteria ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - chemistry ; Bacteriocins ; Bacteriocins - biosynthesis ; Bacteriocins - chemistry ; Biological Sciences ; Biosynthesis ; Chemical synthesis ; Cytotoxins ; Dehydration ; Enzymes ; Factor Xa - metabolism ; Genomes ; Genomics ; Iodoacetamide - chemistry ; Iodoacetamide - metabolism ; Molecular Sequence Data ; Peptides ; Protein Engineering ; Proteins ; Sequence Alignment ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2006-11, Vol.103 (46), p.17243-17248</ispartof><rights>Copyright 2006 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Nov 14, 2006</rights><rights>2006 by The National Academy of Sciences of the USA 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-f23e9c9442452f677b4079d64936a732d02d7fe80d264f59644c91145eaa6fe03</citedby><cites>FETCH-LOGICAL-c530t-f23e9c9442452f677b4079d64936a732d02d7fe80d264f59644c91145eaa6fe03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/103/46.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30052417$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30052417$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829,58593,58826</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17085596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McClerren, Amanda L.</creatorcontrib><creatorcontrib>Cooper, Lisa E.</creatorcontrib><creatorcontrib>Quan, Chao</creatorcontrib><creatorcontrib>Thomas, Paul M.</creatorcontrib><creatorcontrib>Kelleher, Neil L.</creatorcontrib><creatorcontrib>van der Donk, Wilfred A.</creatorcontrib><title>Discovery and in vitro Biosynthesis of Haloduracin, a Two-Component Lantibiotic</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Lantibiotics are ribosomally synthesized peptides that undergo posttranslational modifications to their mature, antimicrobial form. They are characterized by the unique amino acids lanthionine and methyllanthionine, introduced by means of dehydration of Ser/Thr residues followed by reaction of the resulting dehydro amino acids with cysteines to form thioether linkages. Two-component lantibiotics use two peptides that are each posttranslationally modified to yield two functionally distinct products that act in synergy to provide bactericidal activity. By using genetic data instead of isolation, a two-component lantibiotic, haloduracin, was identified in the genome of the Gram-positive alkaliphilic bacterium Bacillus halodurans C-125. We show that heterologously expressed and purified precursor peptides HalA1 and HalA2 are processed by the purified modification enzymes HalM1 and HalM2 in an in vitro reconstitution of the biosynthesis of a two-component lantibiotic. The activity of each HalM enzyme is substratespecific, and the assay products exhibit antimicrobial activity after removal of their leader sequences at an engineered Factor Xa cleavage site, indicating that correct thioether formation has occurred. Haloduracin's biological activity depends on the presence of both modified peptides. The structures of the two mature haloduracin peptides Halα and Halβ were investigated, indicating that they have similarities as well as some distinct differences compared with other two-component lantibiotics.</description><subject>Acetoacetates - metabolism</subject><subject>Adducts</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Antibiotics</subject><subject>Antimicrobials</subject><subject>Bacillus - chemistry</subject><subject>Bacillus - genetics</subject><subject>Bacillus - metabolism</subject><subject>Bacillus halodurans</subject><subject>Bacteria</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacteriocins</subject><subject>Bacteriocins - biosynthesis</subject><subject>Bacteriocins - chemistry</subject><subject>Biological Sciences</subject><subject>Biosynthesis</subject><subject>Chemical synthesis</subject><subject>Cytotoxins</subject><subject>Dehydration</subject><subject>Enzymes</subject><subject>Factor Xa - metabolism</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Iodoacetamide - chemistry</subject><subject>Iodoacetamide - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Peptides</subject><subject>Protein Engineering</subject><subject>Proteins</subject><subject>Sequence Alignment</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkc1vEzEQxS0EoqFw7onK4oBUiW3HH7teX5BoWihSpF7K2XJ27dbRxg62NzT_fR0laqAX5MMc_Js3b-YhdELgnIBgFyuv0zk05bUtAfYKTQhIUjVcwms0AaCiajnlR-hdSgsAkHULb9EREdDWtWwm6PbKpS6sTdxg7XvsPF67HAO-dCFtfH4wySUcLL7RQ-jHqDvnv2CN7_6EahqWq-CNz3imfXZzF7Lr3qM3Vg_JfNjXY_Tr-_Xd9Kaa3f74Of02q7qaQa4sZUZ2khdvNbWNEHMOQvbFN2u0YLQH2gtrWuhpw21xynknCeG10bqxBtgx-rrTXY3zpem7YiPqQa2iW-q4UUE79e-Pdw_qPqwVaWspiSgCn_cCMfweTcpqWS5hhkF7E8akiGSyzJUF_PQCXIQx-rKcokAYb4ngBbrYQV0MKUVjn50QUNuk1DYpdUiqdJz-vcCB30dTALwHtp0HOaZ4UyjKtxpn_0GUHYchm8dc2I87dpFyiM8wA6gpL_d4AnwAsSQ</recordid><startdate>20061114</startdate><enddate>20061114</enddate><creator>McClerren, Amanda L.</creator><creator>Cooper, Lisa E.</creator><creator>Quan, Chao</creator><creator>Thomas, Paul M.</creator><creator>Kelleher, Neil L.</creator><creator>van der Donk, Wilfred A.</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20061114</creationdate><title>Discovery and in vitro Biosynthesis of Haloduracin, a Two-Component Lantibiotic</title><author>McClerren, Amanda L. ; 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A.L.M., L.E.C., and W.A.v.d.D. designed research; A.L.M., L.E.C., C.Q., and P.M.T. performed research; N.L.K. and W.A.v.d.D. analyzed data; and A.L.M., L.E.C., and W.A.v.d.D. wrote the paper.</notes><notes>Edited by Christopher T. Walsh, Harvard Medical School, Boston, MA, and approved September 25, 2006</notes><abstract>Lantibiotics are ribosomally synthesized peptides that undergo posttranslational modifications to their mature, antimicrobial form. They are characterized by the unique amino acids lanthionine and methyllanthionine, introduced by means of dehydration of Ser/Thr residues followed by reaction of the resulting dehydro amino acids with cysteines to form thioether linkages. Two-component lantibiotics use two peptides that are each posttranslationally modified to yield two functionally distinct products that act in synergy to provide bactericidal activity. By using genetic data instead of isolation, a two-component lantibiotic, haloduracin, was identified in the genome of the Gram-positive alkaliphilic bacterium Bacillus halodurans C-125. We show that heterologously expressed and purified precursor peptides HalA1 and HalA2 are processed by the purified modification enzymes HalM1 and HalM2 in an in vitro reconstitution of the biosynthesis of a two-component lantibiotic. The activity of each HalM enzyme is substratespecific, and the assay products exhibit antimicrobial activity after removal of their leader sequences at an engineered Factor Xa cleavage site, indicating that correct thioether formation has occurred. Haloduracin's biological activity depends on the presence of both modified peptides. The structures of the two mature haloduracin peptides Halα and Halβ were investigated, indicating that they have similarities as well as some distinct differences compared with other two-component lantibiotics.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>17085596</pmid><doi>10.1073/pnas.0606088103</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetoacetates - metabolism Adducts Amino Acid Sequence Amino acids Antibiotics Antimicrobials Bacillus - chemistry Bacillus - genetics Bacillus - metabolism Bacillus halodurans Bacteria Bacterial Proteins - biosynthesis Bacterial Proteins - chemistry Bacteriocins Bacteriocins - biosynthesis Bacteriocins - chemistry Biological Sciences Biosynthesis Chemical synthesis Cytotoxins Dehydration Enzymes Factor Xa - metabolism Genomes Genomics Iodoacetamide - chemistry Iodoacetamide - metabolism Molecular Sequence Data Peptides Protein Engineering Proteins Sequence Alignment Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Discovery and in vitro Biosynthesis of Haloduracin, a Two-Component Lantibiotic |
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