A temperature‐jump apparatus for fluorescence measurements

A new instrument has been designed for the recording of chemical relaxation phenomena induced by a temperature jump, using changes in the nonpolarized and polarized emission of fluorescent molecules. The relative features of techniques for the measurements of light absorption and light emission are...

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Bibliographic Details
Published in:Review of scientific instruments 1974-04, Vol.45 (4), p.580-588
Main Authors: Rigler, Rudolf, Rabl, Carl‐Roland, Jovin, Thomas M.
Format: Article
Language:English
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Summary:A new instrument has been designed for the recording of chemical relaxation phenomena induced by a temperature jump, using changes in the nonpolarized and polarized emission of fluorescent molecules. The relative features of techniques for the measurements of light absorption and light emission are discussed specifically in regard to the signal‐to‐noise ratios of the measured signals and to problems related to the detection of polarized emission. Examples are given to demonstrate both the performance of the instrument as well as the usefulness of fluorescence measurements in chemical relaxation experiments.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.1686689