The inhibitory effect of chlorogenic acid on oxidative stress and apoptosis induced by PM 2.5 in HaCaT keratinocytes

Abstract Exposure to fine particulate matter with an aerodynamic diameter of less than 2.5 μm (PM 2.5 ) can cause oxidative damage and apoptosis in the human skin. Chlorogenic acid (CGA) is a bioactive polyphenolic compound with antioxidant, antifungal, and antiviral properties. The objective of thi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biochemical and molecular toxicology 2024-09, Vol.38 (9)
Main Authors: Herath, Herath Mudiyanselage Udari Lakmini, Piao, Mei Jing, Kang, Kyoung Ah, Fernando, Pincha Devage Sameera Madushan, Kang, Hee Kyoung, Koh, Young Sang, Hyun, Jin Won
Format: Article
Language:English
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-crossref_primary_10_1002_jbt_238063
container_end_page
container_issue 9
container_start_page
container_title Journal of biochemical and molecular toxicology
container_volume 38
creator Herath, Herath Mudiyanselage Udari Lakmini
Piao, Mei Jing
Kang, Kyoung Ah
Fernando, Pincha Devage Sameera Madushan
Kang, Hee Kyoung
Koh, Young Sang
Hyun, Jin Won
description Abstract Exposure to fine particulate matter with an aerodynamic diameter of less than 2.5 μm (PM 2.5 ) can cause oxidative damage and apoptosis in the human skin. Chlorogenic acid (CGA) is a bioactive polyphenolic compound with antioxidant, antifungal, and antiviral properties. The objective of this study was to identify the ameliorating impact of CGA that might protect human HaCaT cells against PM 2.5 . CGA significantly scavenged the reactive oxygen species (ROS) generated by PM 2.5 , attenuated oxidative cellular/organelle damage, mitochondrial membrane depolarization, and suppressed cytochrome c release into the cytosol. The application of CGA led to a reduction in the expression levels of Bcl‐2‐associated X protein, caspase‐9, and caspase‐3, while simultaneously increasing the expression of B‐cell lymphoma 2. In addition, CGA was able to reverse the decrease in cell viability caused by PM 2.5 via the inhibition of extracellular signal‐regulated kinase (ERK). This effect was further confirmed by the use of the mitogen‐activated protein kinase kinase inhibitor, which acted upstream of ERK. In conclusion, CGA protected keratinocytes from mitochondrial damage and apoptosis via ameliorating PM 2.5 ‐induced oxidative stress and ERK activation.
doi_str_mv 10.1002/jbt.23806
format article
fullrecord <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_1002_jbt_23806</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1002_jbt_23806</sourcerecordid><originalsourceid>FETCH-crossref_primary_10_1002_jbt_238063</originalsourceid><addsrcrecordid>eNqVj81OwzAQhC0EEuXnwBvslUPCOiUpOVegXpB6yN1y7DVxKdnIaxB5e0LFC3Ca0WhmpE-pO42lRqweDn0uq_UTNmdqpbFtC3xs9PnJ10XTbPBSXYkcELFuN_VK5W4giOMQ-5g5zUAhkMvAAdxw5MRvNEYH1kUPPAJ_R29z_CKQnEgE7OjBTjxllijLj_905KGfYf8KVVkvCezs1nbwTmkZjuzmTHKjLoI9Ct3-6bW6f3nutrvCJRZJFMyU4odNs9FofrHMgmVOWOv_dH8A9XpUuQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>The inhibitory effect of chlorogenic acid on oxidative stress and apoptosis induced by PM 2.5 in HaCaT keratinocytes</title><source>Wiley-Blackwell Journals</source><creator>Herath, Herath Mudiyanselage Udari Lakmini ; Piao, Mei Jing ; Kang, Kyoung Ah ; Fernando, Pincha Devage Sameera Madushan ; Kang, Hee Kyoung ; Koh, Young Sang ; Hyun, Jin Won</creator><creatorcontrib>Herath, Herath Mudiyanselage Udari Lakmini ; Piao, Mei Jing ; Kang, Kyoung Ah ; Fernando, Pincha Devage Sameera Madushan ; Kang, Hee Kyoung ; Koh, Young Sang ; Hyun, Jin Won</creatorcontrib><description>Abstract Exposure to fine particulate matter with an aerodynamic diameter of less than 2.5 μm (PM 2.5 ) can cause oxidative damage and apoptosis in the human skin. Chlorogenic acid (CGA) is a bioactive polyphenolic compound with antioxidant, antifungal, and antiviral properties. The objective of this study was to identify the ameliorating impact of CGA that might protect human HaCaT cells against PM 2.5 . CGA significantly scavenged the reactive oxygen species (ROS) generated by PM 2.5 , attenuated oxidative cellular/organelle damage, mitochondrial membrane depolarization, and suppressed cytochrome c release into the cytosol. The application of CGA led to a reduction in the expression levels of Bcl‐2‐associated X protein, caspase‐9, and caspase‐3, while simultaneously increasing the expression of B‐cell lymphoma 2. In addition, CGA was able to reverse the decrease in cell viability caused by PM 2.5 via the inhibition of extracellular signal‐regulated kinase (ERK). This effect was further confirmed by the use of the mitogen‐activated protein kinase kinase inhibitor, which acted upstream of ERK. In conclusion, CGA protected keratinocytes from mitochondrial damage and apoptosis via ameliorating PM 2.5 ‐induced oxidative stress and ERK activation.</description><identifier>ISSN: 1095-6670</identifier><identifier>EISSN: 1099-0461</identifier><identifier>DOI: 10.1002/jbt.23806</identifier><language>eng</language><ispartof>Journal of biochemical and molecular toxicology, 2024-09, Vol.38 (9)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-crossref_primary_10_1002_jbt_238063</cites><orcidid>0000-0002-3403-3762</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids></links><search><creatorcontrib>Herath, Herath Mudiyanselage Udari Lakmini</creatorcontrib><creatorcontrib>Piao, Mei Jing</creatorcontrib><creatorcontrib>Kang, Kyoung Ah</creatorcontrib><creatorcontrib>Fernando, Pincha Devage Sameera Madushan</creatorcontrib><creatorcontrib>Kang, Hee Kyoung</creatorcontrib><creatorcontrib>Koh, Young Sang</creatorcontrib><creatorcontrib>Hyun, Jin Won</creatorcontrib><title>The inhibitory effect of chlorogenic acid on oxidative stress and apoptosis induced by PM 2.5 in HaCaT keratinocytes</title><title>Journal of biochemical and molecular toxicology</title><description>Abstract Exposure to fine particulate matter with an aerodynamic diameter of less than 2.5 μm (PM 2.5 ) can cause oxidative damage and apoptosis in the human skin. Chlorogenic acid (CGA) is a bioactive polyphenolic compound with antioxidant, antifungal, and antiviral properties. The objective of this study was to identify the ameliorating impact of CGA that might protect human HaCaT cells against PM 2.5 . CGA significantly scavenged the reactive oxygen species (ROS) generated by PM 2.5 , attenuated oxidative cellular/organelle damage, mitochondrial membrane depolarization, and suppressed cytochrome c release into the cytosol. The application of CGA led to a reduction in the expression levels of Bcl‐2‐associated X protein, caspase‐9, and caspase‐3, while simultaneously increasing the expression of B‐cell lymphoma 2. In addition, CGA was able to reverse the decrease in cell viability caused by PM 2.5 via the inhibition of extracellular signal‐regulated kinase (ERK). This effect was further confirmed by the use of the mitogen‐activated protein kinase kinase inhibitor, which acted upstream of ERK. In conclusion, CGA protected keratinocytes from mitochondrial damage and apoptosis via ameliorating PM 2.5 ‐induced oxidative stress and ERK activation.</description><issn>1095-6670</issn><issn>1099-0461</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqVj81OwzAQhC0EEuXnwBvslUPCOiUpOVegXpB6yN1y7DVxKdnIaxB5e0LFC3Ca0WhmpE-pO42lRqweDn0uq_UTNmdqpbFtC3xs9PnJ10XTbPBSXYkcELFuN_VK5W4giOMQ-5g5zUAhkMvAAdxw5MRvNEYH1kUPPAJ_R29z_CKQnEgE7OjBTjxllijLj_905KGfYf8KVVkvCezs1nbwTmkZjuzmTHKjLoI9Ct3-6bW6f3nutrvCJRZJFMyU4odNs9FofrHMgmVOWOv_dH8A9XpUuQ</recordid><startdate>202409</startdate><enddate>202409</enddate><creator>Herath, Herath Mudiyanselage Udari Lakmini</creator><creator>Piao, Mei Jing</creator><creator>Kang, Kyoung Ah</creator><creator>Fernando, Pincha Devage Sameera Madushan</creator><creator>Kang, Hee Kyoung</creator><creator>Koh, Young Sang</creator><creator>Hyun, Jin Won</creator><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-3403-3762</orcidid></search><sort><creationdate>202409</creationdate><title>The inhibitory effect of chlorogenic acid on oxidative stress and apoptosis induced by PM 2.5 in HaCaT keratinocytes</title><author>Herath, Herath Mudiyanselage Udari Lakmini ; Piao, Mei Jing ; Kang, Kyoung Ah ; Fernando, Pincha Devage Sameera Madushan ; Kang, Hee Kyoung ; Koh, Young Sang ; Hyun, Jin Won</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-crossref_primary_10_1002_jbt_238063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herath, Herath Mudiyanselage Udari Lakmini</creatorcontrib><creatorcontrib>Piao, Mei Jing</creatorcontrib><creatorcontrib>Kang, Kyoung Ah</creatorcontrib><creatorcontrib>Fernando, Pincha Devage Sameera Madushan</creatorcontrib><creatorcontrib>Kang, Hee Kyoung</creatorcontrib><creatorcontrib>Koh, Young Sang</creatorcontrib><creatorcontrib>Hyun, Jin Won</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of biochemical and molecular toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herath, Herath Mudiyanselage Udari Lakmini</au><au>Piao, Mei Jing</au><au>Kang, Kyoung Ah</au><au>Fernando, Pincha Devage Sameera Madushan</au><au>Kang, Hee Kyoung</au><au>Koh, Young Sang</au><au>Hyun, Jin Won</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The inhibitory effect of chlorogenic acid on oxidative stress and apoptosis induced by PM 2.5 in HaCaT keratinocytes</atitle><jtitle>Journal of biochemical and molecular toxicology</jtitle><date>2024-09</date><risdate>2024</risdate><volume>38</volume><issue>9</issue><issn>1095-6670</issn><eissn>1099-0461</eissn><abstract>Abstract Exposure to fine particulate matter with an aerodynamic diameter of less than 2.5 μm (PM 2.5 ) can cause oxidative damage and apoptosis in the human skin. Chlorogenic acid (CGA) is a bioactive polyphenolic compound with antioxidant, antifungal, and antiviral properties. The objective of this study was to identify the ameliorating impact of CGA that might protect human HaCaT cells against PM 2.5 . CGA significantly scavenged the reactive oxygen species (ROS) generated by PM 2.5 , attenuated oxidative cellular/organelle damage, mitochondrial membrane depolarization, and suppressed cytochrome c release into the cytosol. The application of CGA led to a reduction in the expression levels of Bcl‐2‐associated X protein, caspase‐9, and caspase‐3, while simultaneously increasing the expression of B‐cell lymphoma 2. In addition, CGA was able to reverse the decrease in cell viability caused by PM 2.5 via the inhibition of extracellular signal‐regulated kinase (ERK). This effect was further confirmed by the use of the mitogen‐activated protein kinase kinase inhibitor, which acted upstream of ERK. In conclusion, CGA protected keratinocytes from mitochondrial damage and apoptosis via ameliorating PM 2.5 ‐induced oxidative stress and ERK activation.</abstract><doi>10.1002/jbt.23806</doi><orcidid>https://orcid.org/0000-0002-3403-3762</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 1095-6670
ispartof Journal of biochemical and molecular toxicology, 2024-09, Vol.38 (9)
issn 1095-6670
1099-0461
language eng
recordid cdi_crossref_primary_10_1002_jbt_23806
source Wiley-Blackwell Journals
title The inhibitory effect of chlorogenic acid on oxidative stress and apoptosis induced by PM 2.5 in HaCaT keratinocytes
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-25T06%3A24%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20inhibitory%20effect%20of%20chlorogenic%20acid%20on%20oxidative%20stress%20and%20apoptosis%20induced%20by%20PM%202.5%20in%20HaCaT%20keratinocytes&rft.jtitle=Journal%20of%20biochemical%20and%20molecular%20toxicology&rft.au=Herath,%20Herath%20Mudiyanselage%20Udari%20Lakmini&rft.date=2024-09&rft.volume=38&rft.issue=9&rft.issn=1095-6670&rft.eissn=1099-0461&rft_id=info:doi/10.1002/jbt.23806&rft_dat=%3Ccrossref%3E10_1002_jbt_23806%3C/crossref%3E%3Cgrp_id%3Ecdi_FETCH-crossref_primary_10_1002_jbt_238063%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true