Loading…
β-Amyloid in Cerebrospinal Fluid: How to Keep It Floating (Not Sticking) by Standardization of Preanalytic Processes and Collection Tubes
Phosphorylated tau (pTau), total tau (tTau), and β-amyloid (Aβ) are established cerebrospinal fluid (CSF) biomarkers used to help diagnose Alzheimer disease. Preanalytic workups of CSF samples lack harmonization, making interlaboratory comparison of these biomarkers challenging. The Aβ adsorbs to sa...
Saved in:
Published in: | The journal of applied laboratory medicine 2021-09, Vol.6 (5), p.1155-1164 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | eng ; nor |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c353t-1d50b51f7695e3510212af3ba4eebe6c5de32bf930a1e51156e121a3af37cd533 |
---|---|
cites | cdi_FETCH-LOGICAL-c353t-1d50b51f7695e3510212af3ba4eebe6c5de32bf930a1e51156e121a3af37cd533 |
container_end_page | 1164 |
container_issue | 5 |
container_start_page | 1155 |
container_title | The journal of applied laboratory medicine |
container_volume | 6 |
creator | Strand, Heidi Garabet, Lamya Bjelke, Börje Sithiravel, Cindhya Hardang, Ingrid Marie Moe, Morten K |
description | Phosphorylated tau (pTau), total tau (tTau), and β-amyloid (Aβ) are established cerebrospinal fluid (CSF) biomarkers used to help diagnose Alzheimer disease. Preanalytic workups of CSF samples lack harmonization, making interlaboratory comparison of these biomarkers challenging. The Aβ adsorbs to sample tubes, yielding underestimated concentrations, and may result in false Alzheimer disease diagnosis. Our primary aim was to compare Aβ recovery across multiple polypropylene tubes and to test the stability of tTau, pTau, and Aβ in the best performing tube.
Eight polypropylene tubes were tested using 3 CSF pools with Aβ concentrations 1000 ng/L. All samples were analyzed in duplicate. Tubes were cut open to assess their different infrared adsorption spectra. Freshly drawn CSF from 14 patients was distributed into 4 Sarstedt 5-mL (no. 63.504.027; Sar5CSF) tubes, left at room temperature for up to 7 days, and analyzed for pTau, tTau, and Aβ by ELISA.
Two Sarstedt 5-mL tubes and a Sarstedt 10-mL (Sar10CSF) tube showed significantly higher Aβ recovery at all 3 concentrations compared with the 5 other tubes. The infrared adsorption spectra of Sar10CSF and Sar5CSF tubes were practically identical, unlike the other tubes. No significant loss of pTau, tTau, and Aβ was observed in CSF left at room temperature for up to 7 days (P > 0.05).
Recovery of Aβ from Sar5CSF tubes is equivalent to Aβ recovery from Sar10CSF tubes. Levels of pTau, tTau, and Aβ were stable for at least 7 days at room temperature but not at 37 °C. |
doi_str_mv | 10.1093/jalm/jfab024 |
format | article |
fullrecord | <record><control><sourceid>proquest_crist</sourceid><recordid>TN_cdi_cristin_nora_10852_92585</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2535833688</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-1d50b51f7695e3510212af3ba4eebe6c5de32bf930a1e51156e121a3af37cd533</originalsourceid><addsrcrecordid>eNo90c9O3DAQBnALURUE3HoGH0FqwH8yTsINraAgUFup9GzZyQR568RbOxHaPkIfhwfpM2Fg6ckzo58-yfoI-cTZKWeNPFsaP5wte2OZKLfIrigrKCpR8u08Q6WKpgS1Qw5SWjLGeC2Ukuwj2ZElg6au1C75---puBjWPriOupEuMKKNIa3caDy98rPrzul1eKRToLeIK3oz5Wswkxsf6PHXMNEfk2t_5e2E2nVezNiZ2Lk_WYSRhp5-j2hy1jqzPIcWU8JEM6OL4D22r-5-tpj2yYfe-IQHm3eP_Ly6vF9cF3ffvtwsLu6KVoKcCt4Bs8D7SjWAEjgTXJheWlMiWlQtdCiF7RvJDEfgHBRywY3Mpmo7kHKPHL3lttGl_BE9hmg0ZzUI3QioIYvjN7GK4feMadKDSy16b0YMc9ICJNRSqrrO9PN7WEgpYq9X0Q0mrnOgfqlIv1SkNxVlfrhJnu2A3X_8Xoh8BpUGjg0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2535833688</pqid></control><display><type>article</type><title>β-Amyloid in Cerebrospinal Fluid: How to Keep It Floating (Not Sticking) by Standardization of Preanalytic Processes and Collection Tubes</title><source>Oxford University Press Journals</source><source>NORA - Norwegian Open Research Archives</source><creator>Strand, Heidi ; Garabet, Lamya ; Bjelke, Börje ; Sithiravel, Cindhya ; Hardang, Ingrid Marie ; Moe, Morten K</creator><creatorcontrib>Strand, Heidi ; Garabet, Lamya ; Bjelke, Börje ; Sithiravel, Cindhya ; Hardang, Ingrid Marie ; Moe, Morten K</creatorcontrib><description>Phosphorylated tau (pTau), total tau (tTau), and β-amyloid (Aβ) are established cerebrospinal fluid (CSF) biomarkers used to help diagnose Alzheimer disease. Preanalytic workups of CSF samples lack harmonization, making interlaboratory comparison of these biomarkers challenging. The Aβ adsorbs to sample tubes, yielding underestimated concentrations, and may result in false Alzheimer disease diagnosis. Our primary aim was to compare Aβ recovery across multiple polypropylene tubes and to test the stability of tTau, pTau, and Aβ in the best performing tube.
Eight polypropylene tubes were tested using 3 CSF pools with Aβ concentrations <500, 500-1000, and >1000 ng/L. All samples were analyzed in duplicate. Tubes were cut open to assess their different infrared adsorption spectra. Freshly drawn CSF from 14 patients was distributed into 4 Sarstedt 5-mL (no. 63.504.027; Sar5CSF) tubes, left at room temperature for up to 7 days, and analyzed for pTau, tTau, and Aβ by ELISA.
Two Sarstedt 5-mL tubes and a Sarstedt 10-mL (Sar10CSF) tube showed significantly higher Aβ recovery at all 3 concentrations compared with the 5 other tubes. The infrared adsorption spectra of Sar10CSF and Sar5CSF tubes were practically identical, unlike the other tubes. No significant loss of pTau, tTau, and Aβ was observed in CSF left at room temperature for up to 7 days (P > 0.05).
Recovery of Aβ from Sar5CSF tubes is equivalent to Aβ recovery from Sar10CSF tubes. Levels of pTau, tTau, and Aβ were stable for at least 7 days at room temperature but not at 37 °C.</description><identifier>ISSN: 2576-9456</identifier><identifier>EISSN: 2475-7241</identifier><identifier>DOI: 10.1093/jalm/jfab024</identifier><identifier>PMID: 34059876</identifier><language>eng ; nor</language><publisher>England</publisher><subject>Alzheimer Disease - diagnosis ; Amyloid beta-Peptides - cerebrospinal fluid ; Biomarkers ; Enzyme-Linked Immunosorbent Assay ; Humans ; Reference Standards</subject><ispartof>The journal of applied laboratory medicine, 2021-09, Vol.6 (5), p.1155-1164</ispartof><rights>American Association for Clinical Chemistry 2021. All rights reserved. For permissions, please email: journals.permissions@oup.com.</rights><rights>info:eu-repo/semantics/openAccess</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-1d50b51f7695e3510212af3ba4eebe6c5de32bf930a1e51156e121a3af37cd533</citedby><cites>FETCH-LOGICAL-c353t-1d50b51f7695e3510212af3ba4eebe6c5de32bf930a1e51156e121a3af37cd533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,26600,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34059876$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Strand, Heidi</creatorcontrib><creatorcontrib>Garabet, Lamya</creatorcontrib><creatorcontrib>Bjelke, Börje</creatorcontrib><creatorcontrib>Sithiravel, Cindhya</creatorcontrib><creatorcontrib>Hardang, Ingrid Marie</creatorcontrib><creatorcontrib>Moe, Morten K</creatorcontrib><title>β-Amyloid in Cerebrospinal Fluid: How to Keep It Floating (Not Sticking) by Standardization of Preanalytic Processes and Collection Tubes</title><title>The journal of applied laboratory medicine</title><addtitle>J Appl Lab Med</addtitle><description>Phosphorylated tau (pTau), total tau (tTau), and β-amyloid (Aβ) are established cerebrospinal fluid (CSF) biomarkers used to help diagnose Alzheimer disease. Preanalytic workups of CSF samples lack harmonization, making interlaboratory comparison of these biomarkers challenging. The Aβ adsorbs to sample tubes, yielding underestimated concentrations, and may result in false Alzheimer disease diagnosis. Our primary aim was to compare Aβ recovery across multiple polypropylene tubes and to test the stability of tTau, pTau, and Aβ in the best performing tube.
Eight polypropylene tubes were tested using 3 CSF pools with Aβ concentrations <500, 500-1000, and >1000 ng/L. All samples were analyzed in duplicate. Tubes were cut open to assess their different infrared adsorption spectra. Freshly drawn CSF from 14 patients was distributed into 4 Sarstedt 5-mL (no. 63.504.027; Sar5CSF) tubes, left at room temperature for up to 7 days, and analyzed for pTau, tTau, and Aβ by ELISA.
Two Sarstedt 5-mL tubes and a Sarstedt 10-mL (Sar10CSF) tube showed significantly higher Aβ recovery at all 3 concentrations compared with the 5 other tubes. The infrared adsorption spectra of Sar10CSF and Sar5CSF tubes were practically identical, unlike the other tubes. No significant loss of pTau, tTau, and Aβ was observed in CSF left at room temperature for up to 7 days (P > 0.05).
Recovery of Aβ from Sar5CSF tubes is equivalent to Aβ recovery from Sar10CSF tubes. Levels of pTau, tTau, and Aβ were stable for at least 7 days at room temperature but not at 37 °C.</description><subject>Alzheimer Disease - diagnosis</subject><subject>Amyloid beta-Peptides - cerebrospinal fluid</subject><subject>Biomarkers</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Humans</subject><subject>Reference Standards</subject><issn>2576-9456</issn><issn>2475-7241</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>3HK</sourceid><recordid>eNo90c9O3DAQBnALURUE3HoGH0FqwH8yTsINraAgUFup9GzZyQR568RbOxHaPkIfhwfpM2Fg6ckzo58-yfoI-cTZKWeNPFsaP5wte2OZKLfIrigrKCpR8u08Q6WKpgS1Qw5SWjLGeC2Ukuwj2ZElg6au1C75---puBjWPriOupEuMKKNIa3caDy98rPrzul1eKRToLeIK3oz5Wswkxsf6PHXMNEfk2t_5e2E2nVezNiZ2Lk_WYSRhp5-j2hy1jqzPIcWU8JEM6OL4D22r-5-tpj2yYfe-IQHm3eP_Ly6vF9cF3ffvtwsLu6KVoKcCt4Bs8D7SjWAEjgTXJheWlMiWlQtdCiF7RvJDEfgHBRywY3Mpmo7kHKPHL3lttGl_BE9hmg0ZzUI3QioIYvjN7GK4feMadKDSy16b0YMc9ICJNRSqrrO9PN7WEgpYq9X0Q0mrnOgfqlIv1SkNxVlfrhJnu2A3X_8Xoh8BpUGjg0</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Strand, Heidi</creator><creator>Garabet, Lamya</creator><creator>Bjelke, Börje</creator><creator>Sithiravel, Cindhya</creator><creator>Hardang, Ingrid Marie</creator><creator>Moe, Morten K</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>3HK</scope></search><sort><creationdate>20210901</creationdate><title>β-Amyloid in Cerebrospinal Fluid: How to Keep It Floating (Not Sticking) by Standardization of Preanalytic Processes and Collection Tubes</title><author>Strand, Heidi ; Garabet, Lamya ; Bjelke, Börje ; Sithiravel, Cindhya ; Hardang, Ingrid Marie ; Moe, Morten K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-1d50b51f7695e3510212af3ba4eebe6c5de32bf930a1e51156e121a3af37cd533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; nor</language><creationdate>2021</creationdate><topic>Alzheimer Disease - diagnosis</topic><topic>Amyloid beta-Peptides - cerebrospinal fluid</topic><topic>Biomarkers</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Humans</topic><topic>Reference Standards</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Strand, Heidi</creatorcontrib><creatorcontrib>Garabet, Lamya</creatorcontrib><creatorcontrib>Bjelke, Börje</creatorcontrib><creatorcontrib>Sithiravel, Cindhya</creatorcontrib><creatorcontrib>Hardang, Ingrid Marie</creatorcontrib><creatorcontrib>Moe, Morten K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>NORA - Norwegian Open Research Archives</collection><jtitle>The journal of applied laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Strand, Heidi</au><au>Garabet, Lamya</au><au>Bjelke, Börje</au><au>Sithiravel, Cindhya</au><au>Hardang, Ingrid Marie</au><au>Moe, Morten K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>β-Amyloid in Cerebrospinal Fluid: How to Keep It Floating (Not Sticking) by Standardization of Preanalytic Processes and Collection Tubes</atitle><jtitle>The journal of applied laboratory medicine</jtitle><addtitle>J Appl Lab Med</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>6</volume><issue>5</issue><spage>1155</spage><epage>1164</epage><pages>1155-1164</pages><issn>2576-9456</issn><eissn>2475-7241</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Phosphorylated tau (pTau), total tau (tTau), and β-amyloid (Aβ) are established cerebrospinal fluid (CSF) biomarkers used to help diagnose Alzheimer disease. Preanalytic workups of CSF samples lack harmonization, making interlaboratory comparison of these biomarkers challenging. The Aβ adsorbs to sample tubes, yielding underestimated concentrations, and may result in false Alzheimer disease diagnosis. Our primary aim was to compare Aβ recovery across multiple polypropylene tubes and to test the stability of tTau, pTau, and Aβ in the best performing tube.
Eight polypropylene tubes were tested using 3 CSF pools with Aβ concentrations <500, 500-1000, and >1000 ng/L. All samples were analyzed in duplicate. Tubes were cut open to assess their different infrared adsorption spectra. Freshly drawn CSF from 14 patients was distributed into 4 Sarstedt 5-mL (no. 63.504.027; Sar5CSF) tubes, left at room temperature for up to 7 days, and analyzed for pTau, tTau, and Aβ by ELISA.
Two Sarstedt 5-mL tubes and a Sarstedt 10-mL (Sar10CSF) tube showed significantly higher Aβ recovery at all 3 concentrations compared with the 5 other tubes. The infrared adsorption spectra of Sar10CSF and Sar5CSF tubes were practically identical, unlike the other tubes. No significant loss of pTau, tTau, and Aβ was observed in CSF left at room temperature for up to 7 days (P > 0.05).
Recovery of Aβ from Sar5CSF tubes is equivalent to Aβ recovery from Sar10CSF tubes. Levels of pTau, tTau, and Aβ were stable for at least 7 days at room temperature but not at 37 °C.</abstract><cop>England</cop><pmid>34059876</pmid><doi>10.1093/jalm/jfab024</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2576-9456 |
ispartof | The journal of applied laboratory medicine, 2021-09, Vol.6 (5), p.1155-1164 |
issn | 2576-9456 2475-7241 |
language | eng ; nor |
recordid | cdi_cristin_nora_10852_92585 |
source | Oxford University Press Journals; NORA - Norwegian Open Research Archives |
subjects | Alzheimer Disease - diagnosis Amyloid beta-Peptides - cerebrospinal fluid Biomarkers Enzyme-Linked Immunosorbent Assay Humans Reference Standards |
title | β-Amyloid in Cerebrospinal Fluid: How to Keep It Floating (Not Sticking) by Standardization of Preanalytic Processes and Collection Tubes |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T21%3A18%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_crist&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%CE%B2-Amyloid%20in%20Cerebrospinal%20Fluid:%20How%20to%20Keep%20It%20Floating%20(Not%20Sticking)%20by%20Standardization%20of%20Preanalytic%20Processes%20and%20Collection%20Tubes&rft.jtitle=The%20journal%20of%20applied%20laboratory%20medicine&rft.au=Strand,%20Heidi&rft.date=2021-09-01&rft.volume=6&rft.issue=5&rft.spage=1155&rft.epage=1164&rft.pages=1155-1164&rft.issn=2576-9456&rft.eissn=2475-7241&rft_id=info:doi/10.1093/jalm/jfab024&rft_dat=%3Cproquest_crist%3E2535833688%3C/proquest_crist%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c353t-1d50b51f7695e3510212af3ba4eebe6c5de32bf930a1e51156e121a3af37cd533%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2535833688&rft_id=info:pmid/34059876&rfr_iscdi=true |