绿茶多酚表没食子儿茶素没食子酸酯（EGCG）体外对抗乙肝病毒复制的能力

研究目的：乙肝是尚未解决的公众健康问题，需要开发新型抗病毒药物。本研究拟从天然植物绿茶中寻找有效、经济、毒副作用低的抗乙肝病毒药物。创新要点：研究方法：重要结论首次以HepG22．2．15细胞为载体，发现EGCG对乙肝病毒HBsAg、HBeAg和HBVDNA的选择性作用。采用酶联免疫吸附实验（ELISA）检测细胞上清中HBsAg和HBeAg的含量，采用反转录聚合酶链反应（RT-PCR）检测上清和细胞内HBVDNA的含量。EGCG的浓度在0．11～0．44μmol／ml（即50～200μg／ml）的范围内能有效抑制HepG22．2．15细胞中HBsAg和HBeAg的分泌，其效果甚至优于浓度为0．...

Full description

Saved in:
Bibliographic Details
Published in:浙江大学学报：B卷英文版 2014, Vol.15 (6), p.533-539
Main Author: Jing-yao PANG Kui-jun ZHAO Jia-bo WANG Zhi-jie MA Xiao-he XIAO
Format: Article
Language:English
Subjects:
2215细胞
EGCG
HepG2
乙肝病毒（HBV）
抗病毒
拉米夫定
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 539
container_issue 6
container_start_page 533
container_title 浙江大学学报：B卷英文版
container_volume 15
creator Jing-yao PANG Kui-jun ZHAO Jia-bo WANG Zhi-jie MA Xiao-he XIAO
description 研究目的：乙肝是尚未解决的公众健康问题，需要开发新型抗病毒药物。本研究拟从天然植物绿茶中寻找有效、经济、毒副作用低的抗乙肝病毒药物。创新要点：研究方法：重要结论首次以HepG22．2．15细胞为载体，发现EGCG对乙肝病毒HBsAg、HBeAg和HBVDNA的选择性作用。采用酶联免疫吸附实验（ELISA）检测细胞上清中HBsAg和HBeAg的含量，采用反转录聚合酶链反应（RT-PCR）检测上清和细胞内HBVDNA的含量。EGCG的浓度在0．11～0．44μmol／ml（即50～200μg／ml）的范围内能有效抑制HepG22．2．15细胞中HBsAg和HBeAg的分泌，其效果甚至优于浓度为0．87μmol／ml（即200μg/ml）的拉米夫定（3TC），并且具有时间和剂量依赖性。同时，EGCG也能抑制胞外HBV DNA的表达。研究结果表明，EGCG具有良好抗病毒活性和低毒副作用，有望开发成为一种新的抗病毒药物。
format article
fullrecord <record><control><sourceid>chongqing</sourceid><recordid>TN_cdi_chongqing_primary_50200148</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>50200148</cqvip_id><sourcerecordid>50200148</sourcerecordid><originalsourceid>FETCH-chongqing_primary_502001483</originalsourceid><addsrcrecordid>eNpjYuA0tDAz0jU0tzBmAbLNzI11DU0tDDkYuIqLswwMTEwMzM04GRKf797_omfb0yWzXrbOerFwxbNNC18unv907YSnLSDx51sWwEVetu542br-_Z4OV3dn9_d7Op_snfx0ybSn63c-65r-ZOfMF01zn09vfbZ-0tMlvU87tj2f1fKiee_Trtk8DKxpiTnFqbxQmptB0c01xNlDNzkjPy-9MDMvPb6gKDM3sagy3tTAyMDA0MTCmBg1AF_iZPM</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>绿茶多酚表没食子儿茶素没食子酸酯（EGCG）体外对抗乙肝病毒复制的能力</title><source>Springer Link</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Jing-yao PANG Kui-jun ZHAO Jia-bo WANG Zhi-jie MA Xiao-he XIAO</creator><creatorcontrib>Jing-yao PANG Kui-jun ZHAO Jia-bo WANG Zhi-jie MA Xiao-he XIAO</creatorcontrib><description>研究目的：乙肝是尚未解决的公众健康问题，需要开发新型抗病毒药物。本研究拟从天然植物绿茶中寻找有效、经济、毒副作用低的抗乙肝病毒药物。创新要点：研究方法：重要结论首次以HepG22．2．15细胞为载体，发现EGCG对乙肝病毒HBsAg、HBeAg和HBVDNA的选择性作用。采用酶联免疫吸附实验（ELISA）检测细胞上清中HBsAg和HBeAg的含量，采用反转录聚合酶链反应（RT-PCR）检测上清和细胞内HBVDNA的含量。EGCG的浓度在0．11～0．44μmol／ml（即50～200μg／ml）的范围内能有效抑制HepG22．2．15细胞中HBsAg和HBeAg的分泌，其效果甚至优于浓度为0．87μmol／ml（即200μg/ml）的拉米夫定（3TC），并且具有时间和剂量依赖性。同时，EGCG也能抑制胞外HBV DNA的表达。研究结果表明，EGCG具有良好抗病毒活性和低毒副作用，有望开发成为一种新的抗病毒药物。</description><identifier>ISSN: 1673-1581</identifier><identifier>EISSN: 1862-1783</identifier><language>eng</language><subject>2215细胞 ; EGCG ; HepG2 ; 乙肝病毒（HBV） ; 抗病毒 ; 拉米夫定</subject><ispartof>浙江大学学报：B卷英文版, 2014, Vol.15 (6), p.533-539</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86281A/86281A.jpg</thumbnail><link.rule.ids>315,786,790,4043</link.rule.ids></links><search><creatorcontrib>Jing-yao PANG Kui-jun ZHAO Jia-bo WANG Zhi-jie MA Xiao-he XIAO</creatorcontrib><title>绿茶多酚表没食子儿茶素没食子酸酯（EGCG）体外对抗乙肝病毒复制的能力</title><title>浙江大学学报：B卷英文版</title><addtitle>Journal of Zhejiang University Science</addtitle><description>研究目的：乙肝是尚未解决的公众健康问题，需要开发新型抗病毒药物。本研究拟从天然植物绿茶中寻找有效、经济、毒副作用低的抗乙肝病毒药物。创新要点：研究方法：重要结论首次以HepG22．2．15细胞为载体，发现EGCG对乙肝病毒HBsAg、HBeAg和HBVDNA的选择性作用。采用酶联免疫吸附实验（ELISA）检测细胞上清中HBsAg和HBeAg的含量，采用反转录聚合酶链反应（RT-PCR）检测上清和细胞内HBVDNA的含量。EGCG的浓度在0．11～0．44μmol／ml（即50～200μg／ml）的范围内能有效抑制HepG22．2．15细胞中HBsAg和HBeAg的分泌，其效果甚至优于浓度为0．87μmol／ml（即200μg/ml）的拉米夫定（3TC），并且具有时间和剂量依赖性。同时，EGCG也能抑制胞外HBV DNA的表达。研究结果表明，EGCG具有良好抗病毒活性和低毒副作用，有望开发成为一种新的抗病毒药物。</description><subject>2215细胞</subject><subject>EGCG</subject><subject>HepG2</subject><subject>乙肝病毒（HBV）</subject><subject>抗病毒</subject><subject>拉米夫定</subject><issn>1673-1581</issn><issn>1862-1783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNpjYuA0tDAz0jU0tzBmAbLNzI11DU0tDDkYuIqLswwMTEwMzM04GRKf797_omfb0yWzXrbOerFwxbNNC18unv907YSnLSDx51sWwEVetu542br-_Z4OV3dn9_d7Op_snfx0ybSn63c-65r-ZOfMF01zn09vfbZ-0tMlvU87tj2f1fKiee_Trtk8DKxpiTnFqbxQmptB0c01xNlDNzkjPy-9MDMvPb6gKDM3sagy3tTAyMDA0MTCmBg1AF_iZPM</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Jing-yao PANG Kui-jun ZHAO Jia-bo WANG Zhi-jie MA Xiao-he XIAO</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope></search><sort><creationdate>2014</creationdate><title>绿茶多酚表没食子儿茶素没食子酸酯（EGCG）体外对抗乙肝病毒复制的能力</title><author>Jing-yao PANG Kui-jun ZHAO Jia-bo WANG Zhi-jie MA Xiao-he XIAO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_502001483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>2215细胞</topic><topic>EGCG</topic><topic>HepG2</topic><topic>乙肝病毒（HBV）</topic><topic>抗病毒</topic><topic>拉米夫定</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jing-yao PANG Kui-jun ZHAO Jia-bo WANG Zhi-jie MA Xiao-he XIAO</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>浙江大学学报：B卷英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jing-yao PANG Kui-jun ZHAO Jia-bo WANG Zhi-jie MA Xiao-he XIAO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>绿茶多酚表没食子儿茶素没食子酸酯（EGCG）体外对抗乙肝病毒复制的能力</atitle><jtitle>浙江大学学报：B卷英文版</jtitle><addtitle>Journal of Zhejiang University Science</addtitle><date>2014</date><risdate>2014</risdate><volume>15</volume><issue>6</issue><spage>533</spage><epage>539</epage><pages>533-539</pages><issn>1673-1581</issn><eissn>1862-1783</eissn><notes>33-1356/Q</notes><notes>Epigallocatechin-3-gallate, Hepatitis B virus （HBV）, Antiviral activity, HepG2 2.2.15, Lamivudine</notes><notes>Although several antiviral drugs and vaccines are available for use against hepatitis B virus （HBV）, hepatitis caused by HBV remains a major public health problem worldwide, which has not yet been resolved, and new anti-HBV drugs are in great demand. The present study was performed to investigate the anti-HBV activity of epigallocatechin- 3-gallate （EGCG）, a natural-origin compound, in HepG2 2.2.15 cells. The antiviral activity of EGCG was examined by detecting the levels of HBsAg and HBeAg in the supematant and extracellular HBV DNA. EGCG effectively suppressed the secretion of HBsAg and HBeAg from HepG2 2.2.15 cells in a dose- and time-dependent manner, and it showed stronger effects at the level of 0.11-0.44 pmol/ml （50-200 μg/ml） than lamivudine （3TC） at 0.87 μmol/ml （200 pg/ml）. EGCG also suppressed the amount of extracellular HBV DNA. The data indicated that EGCG possessed anti-HBV activity and suggested the potential of EGCG as an effective anti-HBV agent with low toxicity.</notes><abstract>研究目的：乙肝是尚未解决的公众健康问题，需要开发新型抗病毒药物。本研究拟从天然植物绿茶中寻找有效、经济、毒副作用低的抗乙肝病毒药物。创新要点：研究方法：重要结论首次以HepG22．2．15细胞为载体，发现EGCG对乙肝病毒HBsAg、HBeAg和HBVDNA的选择性作用。采用酶联免疫吸附实验（ELISA）检测细胞上清中HBsAg和HBeAg的含量，采用反转录聚合酶链反应（RT-PCR）检测上清和细胞内HBVDNA的含量。EGCG的浓度在0．11～0．44μmol／ml（即50～200μg／ml）的范围内能有效抑制HepG22．2．15细胞中HBsAg和HBeAg的分泌，其效果甚至优于浓度为0．87μmol／ml（即200μg/ml）的拉米夫定（3TC），并且具有时间和剂量依赖性。同时，EGCG也能抑制胞外HBV DNA的表达。研究结果表明，EGCG具有良好抗病毒活性和低毒副作用，有望开发成为一种新的抗病毒药物。</abstract></addata></record>
fulltext fulltext
identifier ISSN: 1673-1581
ispartof 浙江大学学报：B卷英文版, 2014, Vol.15 (6), p.533-539
issn 1673-1581
1862-1783
language eng
recordid cdi_chongqing_primary_50200148
source Springer Link; PubMed Central; Free Full-Text Journals in Chemistry
subjects 2215细胞
EGCG
HepG2
乙肝病毒（HBV）
抗病毒
拉米夫定
title 绿茶多酚表没食子儿茶素没食子酸酯（EGCG）体外对抗乙肝病毒复制的能力
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T04%3A47%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-chongqing&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%E7%BB%BF%E8%8C%B6%E5%A4%9A%E9%85%9A%E8%A1%A8%E6%B2%A1%E9%A3%9F%E5%AD%90%E5%84%BF%E8%8C%B6%E7%B4%A0%E6%B2%A1%E9%A3%9F%E5%AD%90%E9%85%B8%E9%85%AF%EF%BC%88EGCG%EF%BC%89%E4%BD%93%E5%A4%96%E5%AF%B9%E6%8A%97%E4%B9%99%E8%82%9D%E7%97%85%E6%AF%92%E5%A4%8D%E5%88%B6%E7%9A%84%E8%83%BD%E5%8A%9B&rft.jtitle=%E6%B5%99%E6%B1%9F%E5%A4%A7%E5%AD%A6%E5%AD%A6%E6%8A%A5%EF%BC%9AB%E5%8D%B7%E8%8B%B1%E6%96%87%E7%89%88&rft.au=Jing-yao%20PANG%20Kui-jun%20ZHAO%20Jia-bo%20WANG%20Zhi-jie%20MA%20Xiao-he%20XIAO&rft.date=2014&rft.volume=15&rft.issue=6&rft.spage=533&rft.epage=539&rft.pages=533-539&rft.issn=1673-1581&rft.eissn=1862-1783&rft_id=info:doi/&rft_dat=%3Cchongqing%3E50200148%3C/chongqing%3E%3Cgrp_id%3Ecdi_FETCH-chongqing_primary_502001483%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_cqvip_id=50200148&rfr_iscdi=true